Axonlab - CRP measurement with Microsemi CRP & Microsemi CRP LC-767

CRP measurement with Microsemi CRP & Microsemi CRP LC-767

  • timer  4.5 Minuten Lesedauer
  • 13. augustus 2021
  • Geschrieben von Jeanette Fehlmann
  • General Practice

Axonlab has been marketing the Microsemi CRP for more than 20 years in the 3rd device generation and is newly marketing the Microsemi CRP LC-767G (4th device generation). We are pleased to offer our customers a thoroughly proven and reliable device which consistently does very well in interlaboratory test reports.

The measurement results of the Microsemi CRP device series from Horiba are consistent at a high level and are high-quality, with a low coefficient of variation – especially also in the case of the CRP parameter.

This stable and very good quality of the results is achieved through a complex technology which justifies the price of the measurement and requires somewhat more time for a precise measurement than other technologies. The advantage: The measurement is very simple. The blood sample can, without preparation, be placed directly in the device and analysed, which also saves time.

A high-quality CRP measurement which is reflected in the quality of the results and the coefficient of variation requires the following steps:

1. CRP-R1 Haemolysis – Lysis of the erythrocytes in whole blood

2. CRP-R2 Buffer – Inhibits antigens except for CRP

3. CRP-R3 Latex reagent agglutination of the CRP around the latex beads

4. Absorption measurement – Spectrophotometric measurement

In the haemolysation of whole blood with the haemolysis active substance (R-1) and the subsequent reaction with the latex reagent coated with anti-human CRP antibodies (R-3) in the presence of stabilisation active substance (R-2), the latex particles agglutinate through the antigen-antibody reaction between the antibodies and the CRP in the sample.

The change in turbidity which occurs as a result of this agglutination is measured using red light and the CRP concentration in the haemolysed sample is determined based on the polynomial calibration curve created beforehand on the basis of standard serum. In addition, this concentration is converted into the CRP concentration in the blood plasma of the test object based on the haematocrit value measured at the same time and displayed.

Why is a low coefficient of variation (CV%) so important?

In order to assess the accuracy of the measurement, the coefficient of variation (CV) is frequently used as a guideline for the reliability in many clinical investigations. For example, as a diagnostic test, biochemical assay, etc.


What is a CV? And what does reliability mean?

Coefficient of variation (CV)

  • It is a measurement of the random variability of a test
  • It is defined as the standard deviation (SD) divided by the mean of the measurement


  • The scope of a test or an instrument measures a variable consistently
  • Reproducibility or stability of a measurement


Important to know

In reality, each clinical test or each instrument represents a certain degree of test variability and is rarely very reliable (Shechtman, 2013). The reliability/reproducibility for the results are essential for a reliable diagnosis and therapy. A low CV value (low variability and high reproducibility) is thus indispensable.


A low CV has the benefit of a faster and more precise diagnosis in order to make the optimal therapeutic decision as a result, with the following advantages:

  • Targeted antibiotic therapy, no unnecessary antibiotic treatments and the resultant disadvantages of antibiotic resistance
  • Faster patient recovery time
  • Cost-effectiveness