innuTaq HOT-A DNA Polymerase

Specifications

Concentration
5 U/µl

(One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTPs into a polynucleotide fraction in 30 minutes at 70 °C.)

PCR Buffer
10× Hot Start Buffer complete; 200 mM Tris-HCl (pH 8.5 at 25 °C); 500 mM KCl; 15 mM MgCl₂
10× Hot Start Buffer without MgCl ₂ ; 200 mM Tris-HCl (pH 8.5 at 25 °C); 500 mM KCl

Enzyme storage buffer
20 mM Tris-HCl; 100 mM KCl; 0.1 mM EDTA; 1 mM DTT; 0.5 % Tween 20; 0.5 % Nonidet P40; 50 % (v/v) Glycerol; pH 8.0 (25 °C)

PS-Mg₂+ solution
MgCl₂ stock solution 25 mM Store at –20 °C, avoid frequent thawing and freezing.