EHEC-verrijkingsbouillon

Enrichment medium for EHEC from stool samples in clinical microbiology

Art.nr. 10034854
Ref.nr. AL0602
Axonbiotech GmbH

270.10

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Meer dan 30 jaar ervaring

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Bacteriegroei en de vorming van Shiga-toxines door EHEC-bacteriën worden gestimuleerd in de EHEC-verrijkingsbouillon. Na de verrijking kunnen Shiga-toxines worden gedetecteerd met een ELISA.

A fundamental problem when isolating EHEC bacteria from stool samples is that apathogenic E. coli, which are resident in the gut, are always present at the same time as EHEC bacteria but in larger numbers and with a wide range of types. Enrichment in a suitable culture medium is therefore necessary to isolate suspected EHEC bacteria. Yet a pre-enrichment or enrichment of the relevant bacteria has also been found to be necessary for finding evidence of Shiga toxins.

Mening van een expert

Dit zeggen onze experts over het product

That is the difference quality makes: the EHEC enrichment broth – ready to use for the precise and rapid EHEC diagnostics required. It isolates EHEC directly from the complex stool matrix by stimulating growth and Shiga toxin formation, ensuring that even weak toxin producers are reliably detected and subsequently clearly identifiable by ELISA. The result is a higher detection rate, fewer repetitions, and faster, more reliable release of findings. The ready-to-use product eliminates the time-consuming preparation of in-house culture media: 100 tubes of 4.5 ml each – open the cap, add the sample, mix, incubate for 18–24 hours at 37 °C, and then analyze directly. Specific and rapid around the clock, the enrichment step increases speed and reliability regardless of the personnel performing the test. The selective formulation with novobiocin, cefsulodin, and mitomycin C suppresses accompanying flora, selectively enriches EHEC, and inductively promotes toxin production – exactly the combination that provides a clear diagnostic signal under complex conditions.

Expert sinds 2013