Bacterial growth and the formation of Shiga toxins by EHEC bacteria are stimulated in the EHEC enrichment broth. After the enrichment Shiga toxins can be detected using an ELISA.
Sugar-Free Agar is a carbohydrate-free medium used for the detection and enumeration of microorganisms that do not undergo specific fermentation processes during the preparation of dairy products (butter, fermented milk and fresh cheeses)