Sugar-Free Agar is a carbohydrate-free medium used for the detection and enumeration of microorganisms that do not undergo specific fermentation processes during the preparation of dairy products (butter, fermented milk and fresh cheeses)
Bacterial growth and the formation of Shiga toxins by EHEC bacteria are stimulated in the EHEC enrichment broth. After the enrichment Shiga toxins can be detected using an ELISA.